Qualitative and quantitative estimation, antioxidant and anti-Parkinson activities of Kalanchoe lanceolata: A novel medicinal plant

The herb Kalanchoe lanceolata, which is widely used in traditional medicine, has showed promise in treating a range of ailments.The present project was arranged to study the antioxidant and anti Parkinson efficacy of Kalanchoe lanceolataextracts against rotenone-induced Parkinson diseases in rats. Fine powder of the plant was extracted with methanol and then fractionated through various solvents with increasing order of polarity. Phytochemical screenings were done using standard protocols and in-vitro antioxidant activities of plant fractions were evaluated using different free radicals. In-vivo anti-Parkinson and oxidative dysfunction experiments were conducted in rats. Results revealed that various fractions possessed flavonoids, alkaloids, terpenoids saponins, tannin, anthraquinon, and phlobatanine, while terpeniods and alkaloids were absent in aqueous fraction. In-vitro antioxidant activities of various fractions of Kalanchoe lanceolata showed that methanol fraction has remarkable scavenging efficacy of 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and superoxide free radicals followed by chloroform fraction. Free radicals produced by 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Hydrogen peroxide (H2O2), and hydroxyl free radicals were considerably scavenged by methanol fraction followed by ethyl acetate fractions. In-vivo study of animal model showed that methanol fraction has significant recovery effects at behavioural, physiological and biochemical level against rotenone induced Parkinson disease. Kalanchoe lanceolata has significantly improved rotenone-induced motor and nonmotor deficits (depression and cognitive impairments), increased antioxidant enzyme activity, and reduced neurotransmitter changes. It has been concluded from the present data that Kalanchoe lanceolataenhances neurotransmitter levels by alleviating oxidative stress and antioxidant enzyme activity, hence improving motor activity, cognitive functioning, and decreasing depressed behavior. These data suggest that Kalanchoe lanceolatamay be a promising medicinal agent for reducing the risk and progression of Parkinson’s disease.